Alginate-combined cholic acid increased insulin secretion of microencapsulated mouse cloned pancreatic β cells

Ther Deliv. 2017 Oct;8(10):833-842. doi: 10.4155/tde-2017-0042.

Abstract

Aim: A semisynthetic primary bile acid (PBA) has exerted hypoglycemic effects in Type 1 diabetic animals, which were hypothesized to be due to its anti-inflammatory and cellular glucose-regulatory effects. Thus, the research purpose aimed to examine antidiabetic effects of a PBA, in terms of cellular inflammation and survival and insulin release, in the context of supporting β-cell delivery and Type 1 diabetic treatment.

Materials & methods: 10 formulations were prepared, five without PBA (control) and five with PBA (test). Formulations were used to microencapsulate pancreatic β cells and the microcapsules were examined for morphology, cell viability, insulin release and inflammation.

Results & conclusion: PBA improved cell viability, insulin release and reduced inflammation in a formulation-dependent manner, which suggests potential use in cell delivery and diabetes treatment. [Formula: see text].

Keywords: artificial cell microencapsulation; bile acid; pancreatic β cells.

MeSH terms

  • Alginates / pharmacology*
  • Animals
  • Cells, Cultured
  • Cholic Acid / pharmacology*
  • Clone Cells
  • Glucuronic Acid / pharmacology
  • Hexuronic Acids / pharmacology
  • Insulin / metabolism*
  • Insulin Secretion
  • Insulin-Secreting Cells / drug effects*
  • Insulin-Secreting Cells / metabolism
  • Mice

Substances

  • Alginates
  • Hexuronic Acids
  • Insulin
  • Glucuronic Acid
  • Cholic Acid