Co-immunoprecipitation for Identifying Protein-Protein Interactions in Borrelia burgdorferi

Henna Iqbal; Darrin R Akins; Melisha R Kenedy

doi:10.1007/978-1-4939-7383-5_4

Co-immunoprecipitation for Identifying Protein-Protein Interactions in Borrelia burgdorferi

Methods Mol Biol. 2018:1690:47-55. doi: 10.1007/978-1-4939-7383-5_4.

Authors

Henna Iqbal¹, Darrin R Akins², Melisha R Kenedy¹

Affiliations

¹ Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.
² Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA. darrin-akins@ouhsc.edu.

Abstract

Co-immunoprecipitation can be utilized to study protein-protein interactions from various environments, cell types, or tissues. Herein, we describe a co-immunoprecipitation protocol that can be used to examine protein complexes found in the pathogenic spirochete Borrelia burgdorferi. The method outlined here has successfully identified known and unknown members of borrelial protein complexes and is an efficient method for studying protein interactions in this pathogenic spirochete.

Keywords: Borrelia; Co-immunoprecipitation; Protein-protein interactions; Spirochetes.

MeSH terms

Animals
Bacterial Proteins / metabolism*
Borrelia burgdorferi / physiology*
Cell Culture Techniques / methods
Humans
Immunoprecipitation / methods*
Lyme Disease / metabolism
Protein Interaction Mapping / methods*
Protein Interaction Maps*

Substances

Bacterial Proteins

Grants and funding

R01 AI059373/AI/NIAID NIH HHS/United States