Draft genome sequence of a thermostable, alkaliphilic α-amylase and protease producing Bacillus amyloliquefaciens strain KCP2

Vimalkumar S Prajapati; Sanket Ray; Jitendra Narayan; Chaitanya C Joshi; Kamlesh C Patel; Ujjval B Trivedi; R M Patel

doi:10.1007/s13205-017-1005-1

Draft genome sequence of a thermostable, alkaliphilic α-amylase and protease producing Bacillus amyloliquefaciens strain KCP2

3 Biotech. 2017 Dec;7(6):372. doi: 10.1007/s13205-017-1005-1. Epub 2017 Oct 13.

Authors

Vimalkumar S Prajapati¹, Sanket Ray², Jitendra Narayan³, Chaitanya C Joshi⁴, Kamlesh C Patel², Ujjval B Trivedi², R M Patel¹

Affiliations

¹ Aspee Shakilam Biotechnology Institute, Navsari Agricultural University, Surat, Gujarat 395 007 India.
² Department of Biosciences, Satellite Campus, Sardar Patel University, Bakrol, Gujarat India.
³ Research Unit in Environmental and Evolutionary Biology, University of Namur, Namur, Belgium.
⁴ Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat India.

Abstract

Bacillus amyloliquefaciens strain KCP2 was isolated from municipal food waste samples collected in Vallabh Vidyanagar, Gujarat, India. Strain KCP2 is noteworthy due to its ability to produce a thermostable, alkaliphilic α-amylase and a protease. These enzymes have importance in several industrial processes including bread making, brewing, starch processing, pharmacy, and textile industries. Whole genome sequencing of strain KCP2 showed that the estimated genome size was 3.9 Mb, the G + C content was 46%, and it coded for 4113 genes.

Keywords: Amylases; Bacillus amyloliquefaciens; Ion torrent; NGS; Protease; Whole genome sequencing.

Publication types

Case Reports