Abstract
We compared the abilities of the six internal RNA segments of two avian influenza viruses, A/Mallard/Alberta/88/76 (H3N8) and A/Mallard/NY/6750/78 (H2N2), to confer attenuation on wild-type human influenza A/Bethesda/1/85 (H3N2) virus in seronegative adult volunteers. Live avian-human influenza A reassortant virus vaccines derived from either avian virus parent were comparable in the following properties: safety, infectivity, immunogenicity, and genetic stability. Since the avian influenza A/Mallard/Alberta/76 virus offered no clear advantage as a donor virus, we will conduct our future evaluations on live influenza A virus reassortants derived from the more extensively characterized avian influenza A/Mallard/NY/78 virus.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Viral / biosynthesis*
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Dose-Response Relationship, Immunologic
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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Genes, Viral
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Hemagglutination Inhibition Tests
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Humans
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Influenza A Virus, H3N2 Subtype*
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Influenza A virus / genetics
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Influenza A virus / immunology*
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Influenza A virus / physiology
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Influenza Vaccines / adverse effects
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Influenza Vaccines / immunology*
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Influenza, Human / prevention & control*
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Vaccines, Attenuated / adverse effects
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Vaccines, Attenuated / immunology
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Vaccines, Synthetic / adverse effects
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Vaccines, Synthetic / immunology
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Virus Replication
Substances
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Antibodies, Viral
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Influenza Vaccines
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Vaccines, Attenuated
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Vaccines, Synthetic