Photodynamic opening of blood-brain barrier

Oxana Semyachkina-Glushkovskaya; Jürgen Kurths; Ekaterina Borisova; Sergei Sokolovski; Vanya Mantareva; Ivan Angelov; Alexander Shirokov; Nikita Navolokin; Natalia Shushunova; Alexander Khorovodov; Maria Ulanova; Madina Sagatova; Ilana Agranivich; Olga Sindeeva; Artem Gekalyuk; Anastasiya Bodrova; Edik Rafailov

doi:10.1364/BOE.8.005040

Photodynamic opening of blood-brain barrier

Biomed Opt Express. 2017 Oct 17;8(11):5040-5048. doi: 10.1364/BOE.8.005040. eCollection 2017 Nov 1.

Authors

Oxana Semyachkina-Glushkovskaya¹, Jürgen Kurths^{1

2

3}, Ekaterina Borisova^{1

4}, Sergei Sokolovski⁵, Vanya Mantareva⁶, Ivan Angelov⁶, Alexander Shirokov⁷, Nikita Navolokin⁸, Natalia Shushunova¹, Alexander Khorovodov¹, Maria Ulanova¹, Madina Sagatova¹, Ilana Agranivich¹, Olga Sindeeva¹, Artem Gekalyuk¹, Anastasiya Bodrova¹, Edik Rafailov⁵

Affiliations

¹ Saratov State University, Interdisciplinary Center of Critical Technologies in Medicine, Astrakhanskaya Str. 83, Saratov 410012, Russia.
² Humboldt University, Physics Department, Newtonstrasse 15, Berlin, Germany.
³ Potsdam Institute for Climate Impact Research, Telegrafenberg A31, Potsdam, Germany.
⁴ Institute of Electronics, Bulgarian Academy of Sciences, Tsarigradsko Chaussee Blvd. 72, Sofia 1784, Bulgaria.
⁵ Optoelectronics and Biomedical Photonics Group, Aston University, Birmingham, B4 7ET, UK.
⁶ Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bontchev str. Bl. 9, 1113 Sofia, Bulgaria.
⁷ Institute of Bioorganic Chemistry, Russian Academy of Sciences, Entusiastov Str. 13, Saratov 410049, Russia.
⁸ Saratov State Medical University, Saratov 410010, Russia.

Abstract

Photodynamic treatment (PDT) causes a significant increase in the permeability of the blood-brain barrier (BBB) in healthy mice. Using different doses of laser radiation (635 nm, 10-40 J/cm²) and photosensitizer (5-aminolevulinic acid - 5-ALA, 20 and 80 mg/kg, i.v.), we found that the optimal PDT for the reversible opening of the BBB is 15 J/cm² and 5-ALA, 20 mg/kg, exhibiting brain tissues recovery 3 days after PDT. Further increases in the laser radiation or 5-ALA doses have no amplifying effect on the BBB permeability, but are associated with severe damage of brain tissues. These results can be an informative platform for further studies of new strategies in brain drug delivery and for better understanding of mechanisms underlying cerebrovascular effects of PDT-related fluorescence guided resection of brain tumor.

Keywords: (170.0170) Medical optics and biotechnology; (170.1470) Blood or tissue constituent monitoring; (170.2655) Functional monitoring and imaging; (170.3880) Medical and biological imaging.