Paper Spray Tandem Mass Spectrometry Based on Molecularly Imprinted Polymer Substrate for Cocaine Analysis in Oral Fluid

J Am Soc Mass Spectrom. 2018 Mar;29(3):566-572. doi: 10.1007/s13361-017-1853-2. Epub 2017 Dec 1.

Abstract

This study proposes a new direct and fast method of analysis employing paper spray mass spectrometry (PS-MS). The paper used in the proposed method was modified with molecularly imprinted polymers (MIP) to create a specific site for cocaine analysis in oral fluid. MIP membrane was successfully synthetized and employed. The developed method showed to be linear in a concentration range from LOQ to 100 ng mL-1. The experimental value of LOQ obtained was 1 ng mL-1. The inter-day and intra-day precision and accuracy of the PS-MS method presented values lower than 15%. The total recoveries were also evaluated. The PS-MS method for the analysis of cocaine in oral fluid showed to be very promising and the validation parameters showed a good correlation with the literature. Graphical abstract ᅟ.

Keywords: Analytical validation; Cocaine; Oral fluid; Paper spray ionization.

Publication types

  • Validation Study

MeSH terms

  • Cocaine / analysis*
  • Equipment Design
  • Humans
  • Illicit Drugs / analysis*
  • Limit of Detection
  • Molecular Imprinting*
  • Paper
  • Polymers / chemistry
  • Saliva / chemistry*
  • Solid Phase Microextraction / instrumentation
  • Solid Phase Microextraction / methods*
  • Substance Abuse Detection / instrumentation
  • Substance Abuse Detection / methods*
  • Tandem Mass Spectrometry / instrumentation
  • Tandem Mass Spectrometry / methods*

Substances

  • Illicit Drugs
  • Polymers
  • Cocaine