Abstract
This study examines the production of five phenolic acids (chlorogenic acid, neochlorogenic acid, ferulic acid, caffeic acid and p-coumaric acid) following over-expression of AtPAP1 transcription factor by four transgenic root clones of Leonurus sibiricus after Agrobacterium rhizogenes transformation. The AtPAP1 expression level was estimated by quantitative real-time PCR. High levels of phenolic acids were found in the transgenic roots of L. sibiricus and were determined by high-performance liquid chromatography-mass spectrometry analysis. Additionally, transgenic roots showed antimicrobial potential and cytotoxic activity on glioma cells in IV grade. Our results suggest that L. sibiricus transformed roots with AtPAP1 gene over-expression may represent a potential source of phenolic acids.
Keywords:
Antimicrobial activity; AtPAP1; Cytotoxic effect; Gene expression; Leonurus sibiricus; Phenolic acids; Transgenic roots.
MeSH terms
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Agrobacterium / genetics
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Anti-Infective Agents / pharmacology*
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Antineoplastic Agents, Phytogenic / pharmacology*
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Arabidopsis Proteins / genetics*
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Arabidopsis Proteins / metabolism
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Caffeic Acids / metabolism
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Cell Line, Tumor
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Chlorogenic Acid / analogs & derivatives
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Chlorogenic Acid / metabolism
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Coumaric Acids / metabolism
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Gene Expression Regulation, Plant
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Glioma / drug therapy
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Humans
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Leonurus / genetics*
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Leonurus / metabolism*
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Metabolic Engineering / methods
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Plant Roots / genetics
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Plant Roots / metabolism
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Plants, Genetically Modified
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Propionates / metabolism
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Quinic Acid / analogs & derivatives
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Quinic Acid / metabolism
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Real-Time Polymerase Chain Reaction
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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Anti-Infective Agents
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Antineoplastic Agents, Phytogenic
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Arabidopsis Proteins
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Caffeic Acids
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Coumaric Acids
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PAP1 protein, Arabidopsis
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Propionates
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Transcription Factors
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Quinic Acid
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Chlorogenic Acid
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ferulic acid
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p-coumaric acid
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5'-O-caffeoylquinic acid
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caffeic acid