Genome sequence analysis of a hypermucoviscous/hypervirulent and MDR CTX-M-15/K19/ST29 Klebsiella pneumoniae isolated from human infection

Pathog Dis. 2017 Dec 29;75(9). doi: 10.1093/femspd/ftx121.

Abstract

The emergence of hypervirulent Klebsiella pneumoniae (hvKP) with multidrug resistance (MDR) profile is a worrisome public health issue. We report the first draft genome sequence of a hypermucoviscous (positive string test) and MDR K. pneumoniae serotype K19, belonging to ST29, isolated from human infection. This strain harboured multiple antimicrobial resistance genes, including blaCTX-M-15, besides yersiniabactin and type 3 fimbriae virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that K. pneumoniae K19/ST29 killed 100% of the larvae at 24 h post-infection, in a similar way to the known hypermucoviscous hvKP K1/ST23 lineage.

Keywords: Brazil; Galleria mellonella; K. pneumoniae; extended-spectrum beta-lactamase; hvKP; whole genome sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Drug Resistance, Multiple, Bacterial
  • Genome, Bacterial*
  • Humans
  • Klebsiella Infections / microbiology*
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Larva / microbiology
  • Larva / physiology
  • Lepidoptera / microbiology
  • Lepidoptera / physiology
  • Male
  • Middle Aged
  • Sequence Analysis, DNA*
  • Survival Analysis
  • Virulence
  • Virulence Factors / genetics
  • beta-Lactamases / metabolism*

Substances

  • Virulence Factors
  • beta-Lactamases