Atrial Fibrillation Promotion in a Rat Model of Rheumatoid Arthritis

J Am Heart Assoc. 2017 Dec 21;6(12):e007320. doi: 10.1161/JAHA.117.007320.

Abstract

Background: The prevalence of atrial fibrillation (AF) is significantly higher in rheumatoid arthritis (RA) patients, but the underlying mechanisms remain poorly understood. The goal of this study was to assess the effects of RA on AF susceptibility and atrial arrhythmogenic remodeling in a rat model of RA.

Methods and results: Collagen-induced arthritis was induced in rats by immunization with type II collagen in Freund's incomplete adjuvant. Among the rats that developed arthritis, AF susceptibility and atrial remodeling were examined 8 weeks after the primary immunization. AF inducibility and duration were substantially increased in collagen-induced arthritis rats, and AF duration was significantly and positively correlated with the serum IL-6 and TNF-α levels. Rats with collagen-induced arthritis showed prolonged atrial conduction time with no changes in the atrial effective refractory period. Atrial conduction delay was accompanied by significantly increased atrial fibrosis. In addition, atrial structural and autonomic remodeling, including left atrial dilation, apoptosis and autophagy of atrial myocytes, and atrial heterogeneous sympathetic hyperinnervation, was observed. Interestingly, we found that collagen-induced arthritis had no significant effects on connexins, Nav1.5, and the main ion channels' protein expressions in atria.

Conclusions: We demonstrated that RA increased AF susceptibility by inducing AF-promoting atrial remodeling. This study may provide insights into mechanisms underlying RA-induced AF and validate a model that is suitable for further mechanistic and therapeutic exploration.

Keywords: atrial fibrillation; atrial remodeling; autonomic remodeling; fibrosis; rheumatoid arthritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arthritis, Rheumatoid / complications*
  • Arthritis, Rheumatoid / physiopathology
  • Atrial Fibrillation / etiology*
  • Atrial Fibrillation / physiopathology
  • Atrial Remodeling / physiology*
  • Autonomic Nervous System / physiopathology
  • Disease Models, Animal
  • Female
  • Heart Conduction System / physiopathology*
  • Heart Rate
  • Rats
  • Rats, Wistar