Simultaneous editing of GABA and glutathione at 7T using semi-LASER localization

Magn Reson Med. 2018 Aug;80(2):474-479. doi: 10.1002/mrm.27044. Epub 2017 Dec 28.

Abstract

Purpose: To demonstrate simultaneous editing of the two most commonly edited and overlapping signals, γ-aminobutyric acid (GABA), and glutathione (GSH), with Hadamard encoding and reconstruction of MEGA-edited spectroscopy (HERMES) using sLASER localization at 7T.

Methods: Density matrix simulations of HERMES at 7T were carried out and compared with phantom experiments. Additional phantom experiments were performed to characterize the echo time (TE) -dependent modulation of GABA- and GSH-edited HERMES spectra at TE of 80-160 ms. In vivo experiments were performed in 10 healthy volunteers, comparing HERMES (11 min) to sequentially acquired MEGA-sLASER detection of GABA and GSH (2 × 11 min).

Results: Simulations of HERMES show GABA- and GSH-edited spectra with negligible levels of crosstalk, and give modest agreement with phantom spectra. The TE series of GABA- and GSH-edited HERMES spectra modulate as a result of T2 relaxation and coupling evolution, with GABA showing a stronger TE-dependence. In vivo HERMES experiments show well-edited GABA and GSH signals. Measured concentrations are not statistically different between HERMES and MEGA-sLASER for GABA (1. 051 ± 0.254 i.u. and 1.053 ± 0.248 i.u; P > 0.985) or GSH (0.300 ± 0.091 i.u. and 0.302 ± 0.093 i.u; P > 0.940).

Conclusion: Simulated, phantom and in vivo measurements of HERMES show excellent segregation of GABA- and GSH-edited signals, and excellent agreement with separately acquired MEGA-sLASER data. HERMES allows two-fold acceleration of editing while maintaining spectral quality compared with sequentially acquired MEGA-sLASER measurements. Magn Reson Med 80:474-479, 2018. © 2017 International Society for Magnetic Resonance in Medicine.

Keywords: 7T; GABA; GSH; HERMES; editing; semi-LASER.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Computer Simulation
  • Female
  • Glutathione / chemistry*
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Male
  • Phantoms, Imaging
  • Proton Magnetic Resonance Spectroscopy / methods*
  • gamma-Aminobutyric Acid / chemistry*

Substances

  • gamma-Aminobutyric Acid
  • Glutathione