Objective: The effects of prenatal exposure to brominated diphenyl ethers-209 to the Influence of male offspring rats hippocampus BDNF potein expression and its mechanism of action. Methods: Pregnant Sprague-Dawley (SD) rats were randomly treated with BDE-209 (100, 300, and 900 mg/kg body weight) or corn oil by gavage on gestational days 6-20. Blood was obtained through heart puncture for thyroid hormone analysis in male rats offspring on PND 60. The hippocampus tissues were excised. The expression levels of BDNF protein were measured by Western blot. Results: 1) In hippocampal tissue, BDNF protein expression concentration ratio relative to the control group (control group concentration of 1) were 0.87 (300 mg/kg dose group) and 0.67 (900 mg/kg) (P<0.01) . 2) Compared to controls, total T4 levels and free T4 levels were significantly decreased in the BDE-209 treated-group (900 mg/kg, 300 mg/kg) (P<0.05) . Total T3 levels in 300 mg/kg group were also significantly decreased compared to the control (P<0.05) . However, no significant difference was observed in 100 mg/kg group (P>0.05) . Conclusion: During 300 and 900 mg/kg dose group of BDE-209 exposure to male offspring BDNF protein expression in rat hippocampus decreased, may be related to its interference with thyroid hormone.
目的： 观察孕期十溴联苯醚（BDE-209）染毒对雄性子代大鼠海马脑原性神经营养因子（BDNF）蛋白表达的影响。 方法： 将怀孕的SD大鼠随机分为BDE-209染毒组（100、300、900）mg/kg和对照组，于妊娠6~20 d，连续15 d每天对暴露组孕鼠进行灌胃染毒，对照组孕鼠给予等容量玉米油。对PND 60子代大鼠（雄性）分离其海马组织，用Western blot检测雄性子代大鼠海马中BDNF蛋白的表达，同时心脏采血检测甲状腺激素水平。 结果： 孕期BDE-209 300和900 mg/kg剂量组的雄性子代大鼠海马组织中BDNF蛋白的表达明显低于对照组和低剂量组（100 mg/kg），差异有统计学意义（P<0.05）。半定量分析发现，300和900 mg/kg剂量组海马组织中BDNF蛋白表达相对对照组的浓度比（对照组浓度为1）分别为0.87和0.67，差异有统计学意义（P<0.01）。与对照组比较，300和900 mg/kg剂量组雄性子代大鼠的T4水平和FT4水平明显下降，差异有统计学意义（P<0.05），900 mg/kg剂量组的T3水平明显低于对照组，差异有统计学意义（P<0.05），100 mg/kg剂量组与对照组的差异无统计学意义（P>0.05）。 结论： 300和900 mg/kg剂量组BDE-209孕期染毒致雄性子代大鼠海马组织中BDNF蛋白表达下降，可能与其干扰甲状腺激素有关。.
Keywords: Brain-derived neurotrophic factor; Brominated diphenyl ethers; Pregnancy, Animal; Rat; Thyroid hormone.