Lower Limbic Metabotropic Glutamate Receptor 5 Availability in Alcohol Dependence

J Nucl Med. 2018 Apr;59(4):682-690. doi: 10.2967/jnumed.117.199422. Epub 2018 Jan 18.

Abstract

Animal studies suggest an important role for the metabotropic glutamate receptor subtype 5 (mGlu5) in the pathophysiology of alcohol dependence, but direct human evidence is lacking. The goal of this study was to investigate cerebral mGlu5 availability in alcohol-dependent subjects versus controls using 18F-3-fluoro-5-[(pyridin-3-yl)ethynyl]benzonitrile (18F-FPEB) PET. Methods: Dynamic 90-min 18F-FPEB scans combined with arterial blood sampling were acquired for 16 recently abstinent alcohol-dependent subjects and 32 age-matched controls. Regional mGlu5 availability was quantified by the 18F-FPEB total distribution volume using both a voxel-by-voxel and a volume-of-interest analysis with partial-volume effect correction. Alcohol consumption within the last 3 mo was assessed by questionnaires and by hair ethyl glucuronide analysis. Craving was assessed using the Desire for Alcohol Questionnaire. Results: mGlu5 availability was lower in mainly limbic regions of alcohol-dependent subjects than in controls (P < 0.05, familywise error-corrected), ranging from 14% in the posterior cingulate cortex to 36% in the caudate nucleus. Lower mGlu5 availability was associated with higher hair ethyl glucuronide levels for most regions and was related to a lower level of craving specifically in the middle frontal gyrus, cingulate cortex, and inferolateral temporal lobe. Conclusion: These findings provide human in vivo evidence that limbic mGlu5 has a role in the pathophysiology of alcohol dependence, possibly involved in a compensatory mechanism helping to reduce craving during abstinence.

Keywords: PET; alcohol dependence; craving; mGlu5.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcoholism / diagnostic imaging
  • Alcoholism / metabolism*
  • Alcoholism / pathology
  • Atrophy / diagnostic imaging
  • Case-Control Studies
  • Female
  • Humans
  • Limbic System / diagnostic imaging
  • Limbic System / metabolism*
  • Limbic System / pathology
  • Male
  • Middle Aged
  • Nitriles
  • Positron-Emission Tomography
  • Pyridines
  • Receptor, Metabotropic Glutamate 5 / metabolism*

Substances

  • 3-fluoro-5-((pyridin-3-yl)ethynyl)benzonitrile
  • Nitriles
  • Pyridines
  • Receptor, Metabotropic Glutamate 5