Generation of a human embryonic stem cell line expressing tetrameric Zoanthus sp. green fluorescent protein: NERCe002-A-1

Xingxiang Duan; Menghan Xie; Yingying Peng; Liang Hu; Juan Yu; Sicong Zeng; Yang Wang; Guangxiu Lu; Ge Lin; Yi Sun

doi:10.1016/j.scr.2018.01.033

Generation of a human embryonic stem cell line expressing tetrameric Zoanthus sp. green fluorescent protein: NERCe002-A-1

Stem Cell Res. 2018 Apr:28:6-10. doi: 10.1016/j.scr.2018.01.033. Epub 2018 Jan 31.

Authors

Xingxiang Duan¹, Menghan Xie¹, Yingying Peng¹, Liang Hu², Juan Yu³, Sicong Zeng⁴, Yang Wang¹, Guangxiu Lu⁵, Ge Lin⁶, Yi Sun⁷

Affiliations

¹ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
² Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China.
³ National Engineering and Research Center of Human Stem Cell, Changsha, China.
⁴ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China.
⁵ National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
⁶ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
⁷ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China. Electronic address: sunyi66@csu.edu.cn.

PMID: 29414419
DOI: 10.1016/j.scr.2018.01.033

Abstract

The human embryonic stem cell (hESC) line NERCe002-A-1 was generated through lentiviral transduction of the original NERCe002-A-1 hESC line with Zoanthus sp. green fluorescent protein (ZsGreen). Cells that expressed ZsGreen showed a >8.6-fold increase in fluorescence intensity compared with that of cells that expressed enhanced green fluorescent protein. The fluorescent hESC line can aid in identification of biological characteristics in vitro and in vivo by tracking cell growth, migration, and differentiation. Characteristic tests confirmed that the NERCe002-A-1 cell line expressed typical markers of pluripotency and had the capability to form the three germ layers in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anthozoa / metabolism*
Cell Culture Techniques / methods*
Cell Line
Green Fluorescent Proteins / metabolism*
Human Embryonic Stem Cells / cytology*
Humans
Male
Mice
Protein Multimerization*

Substances

Green Fluorescent Proteins