Aspidosperma pyrifolium Mart: neuroprotective, antioxidant and anti-inflammatory effects in a Parkinson's disease model in rats

J Pharm Pharmacol. 2018 Jun;70(6):787-796. doi: 10.1111/jphp.12866. Epub 2018 Feb 28.

Abstract

Objectives: Aspidosperma species are used for several diseases, especially for malaria in Brazil. Although the genus is object of pharmacological studies, almost none are found on Aspidosperma pyrifolium. We investigate neuroprotective, antioxidant and anti-inflammatory properties of the APSE-Aq fraction (benzoic acid glycosylated derivative) on Parkinson's disease model.

Methods: Male Wistar rats were subjected to a 6-hydroxydopamine injection into the right striatum and treated or not with APSE-Aq (100 or 200 mg/kg, p.o.). The sham-operated group was injected with saline. Two weeks later, animals were subjected to behavioural, neurochemical and immunohistochemical evaluation. The data were analysed by ANOVA and Tukey test.

Key findings: The APSE-Aq-treated group shows a partial recovery of behavioural changes as compared with the untreated-6-hydroxydopamine group. A partial recovery was also observed in nitrite contents and lipid peroxidation. APSE-Aq treatments significantly reversed decreases in striatal dopamine and metabolites in the untreated 6-hydroxydopamine group. Immunostainings for markers as tyrosine hydroxylase and dopamine transporter decreased in the untreated 6-hydroxydopamine group and values recovered after APSE-Aq treatments. Similar data were seen for TNF-alpha.

Conclusion: APSE-Aq presents neuroprotective, antioxidant and anti-inflammatory activities. Considering that APSE-Aq is chemically related to salicylic acid, it may act on similar targets.

Keywords: Aspidosperma species; anti-inflammatory action; antioxidant effect; neuroprotection.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants / pharmacology*
  • Aspidosperma / chemistry*
  • Behavior, Animal / drug effects
  • Corpus Striatum / metabolism
  • Disease Models, Animal
  • Dopamine / metabolism
  • Dopamine Plasma Membrane Transport Proteins / metabolism
  • Lipid Peroxidation / drug effects
  • Male
  • Neuroprotective Agents / pharmacology*
  • Nitrites / metabolism
  • Oxidopamine / metabolism
  • Parkinson Disease / drug therapy*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Rats
  • Seeds / chemistry
  • Tumor Necrosis Factor-alpha / metabolism
  • Tyrosine 3-Monooxygenase / metabolism

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Dopamine Plasma Membrane Transport Proteins
  • Neuroprotective Agents
  • Nitrites
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • Oxidopamine
  • Tyrosine 3-Monooxygenase
  • Dopamine