Sensitive Detection of Single-Cell Secreted H2O2 by Integrating a Microfluidic Droplet Sensor and Au Nanoclusters

Rui Shen; Peipei Liu; Yiqiu Zhang; Zhao Yu; Xuyue Chen; Lu Zhou; Baoqing Nie; Anna Żaczek; Jian Chen; Jian Liu

doi:10.1021/acs.analchem.7b04798

Sensitive Detection of Single-Cell Secreted H₂O₂ by Integrating a Microfluidic Droplet Sensor and Au Nanoclusters

Anal Chem. 2018 Apr 3;90(7):4478-4484. doi: 10.1021/acs.analchem.7b04798. Epub 2018 Mar 14.

Authors

Rui Shen¹, Peipei Liu¹, Yiqiu Zhang¹, Zhao Yu¹, Xuyue Chen¹, Lu Zhou¹, Baoqing Nie², Anna Żaczek³, Jian Chen¹, Jian Liu¹

Affiliations

¹ Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices , Soochow University , 199 Ren Ai Road, Suzhou Industrial Park , Suzhou , Jiangsu 215123 , China.
² School of Electronic and Information Engineering , Soochow University , Suzhou , Jiangsu 215123 , China.
³ Medical Biotechnology Department, Intercollegiate Faculty of Biotechnology , University of Gdańsk and Medical University of Gdańsk , Dębinki 1 , Gdańsk , 80-211 , Poland.

PMID: 29510622
DOI: 10.1021/acs.analchem.7b04798

Abstract

As an important signaling molecule, hydrogen peroxide (H₂O₂) secreted externally by the cells influences cell migration, immunity generation, and cellular communications. Herein, we have developed a microfluidic approach with droplets in combination with Au nanoclusters for the sensitive detection of H₂O₂ secreted by a single cell. Isolated in the ultrasmall volume (4.2 nL) of a microdroplet, single-cell secreted H₂O₂ can initiate dramatic fluorescence changes of horseradish peroxidase-Au nanoclusters. We have demonstrated an ultrahigh sensitivity (200-400 attomole H₂O₂ directly measured from a single cell) with good specificity. It offers a useful research tool to study the cell-to-cell differences of H₂O₂ secretion at the single-cell level.

Publication types

Research Support, Non-U.S. Gov't