A new selective broth enrichment automated method for detection of carbapenem-resistant Enterobacteriaceae from rectal swabs

Fabio Arena; Tommaso Giani; Alberto Antonelli; Olga Lorenza Colavecchio; Patrizia Pecile; Bruno Viaggi; Riccardo Favilli; Gian Maria Rossolini

doi:10.1016/j.mimet.2018.03.002

A new selective broth enrichment automated method for detection of carbapenem-resistant Enterobacteriaceae from rectal swabs

J Microbiol Methods. 2018 Apr:147:66-68. doi: 10.1016/j.mimet.2018.03.002. Epub 2018 Mar 7.

Authors

Fabio Arena¹, Tommaso Giani², Alberto Antonelli³, Olga Lorenza Colavecchio³, Patrizia Pecile⁴, Bruno Viaggi⁵, Riccardo Favilli⁶, Gian Maria Rossolini⁷

Affiliations

¹ Department of Medical Biotechnologies, University of Siena, Siena, Italy. Electronic address: arena2@unisi.it.
² Department of Medical Biotechnologies, University of Siena, Siena, Italy.
³ Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
⁴ Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy.
⁵ Neuro Intensive Care Unit, Florence Careggi University Hospital, Florence, Italy.
⁶ Alifax s.r.l., Polverara, Italy.
⁷ Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy.

PMID: 29524455
DOI: 10.1016/j.mimet.2018.03.002

Abstract

We evaluated the performance of an automated, rapid (TAT 4-8 h), liquid-culture method for carbapenem-resistant Enterobacteriaceae (CRE) detection from rectal swabs, in a setting of KPC-producing Klebsiella pneumoniae endemicity. With 600 samples (22 positive for CRE, 3.7%), the system sensitivity and specificity, at 8 h, were 100% and 99.2%, respectively.

Keywords: Carbapenemase; Carriers; Colonization; Italy; Laboratory automation; Screening.

MeSH terms

Anti-Bacterial Agents / pharmacology
Automation, Laboratory / methods*
Bacterial Load
Bacterial Proteins / isolation & purification
Bacterial Typing Techniques / methods*
Carbapenem-Resistant Enterobacteriaceae / drug effects
Carbapenem-Resistant Enterobacteriaceae / growth & development*
Carbapenem-Resistant Enterobacteriaceae / isolation & purification*
Culture Media / chemistry*
Enterobacteriaceae Infections / diagnosis
Enterobacteriaceae Infections / microbiology
Humans
Italy
Microbial Sensitivity Tests
Sensitivity and Specificity
Time Factors
beta-Lactamases / isolation & purification

Substances

Anti-Bacterial Agents
Bacterial Proteins
Culture Media
beta-Lactamases
carbapenemase