Identification of peptides released from hot water insoluble fraction of edible bird's nest under simulated gastro-intestinal conditions

Hang-Kin Kong; Ka-Hing Wong; Samuel Chun-Lap Lo

doi:10.1016/j.foodres.2016.04.002

Identification of peptides released from hot water insoluble fraction of edible bird's nest under simulated gastro-intestinal conditions

Food Res Int. 2016 Jul:85:19-25. doi: 10.1016/j.foodres.2016.04.002. Epub 2016 Apr 16.

Authors

Hang-Kin Kong¹, Ka-Hing Wong², Samuel Chun-Lap Lo³

Affiliations

¹ Food Safety and Technology Research Center, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong; Shenzhen Key Laboratory for Food Biological Safety Control, PolyU Shenzhen Research Institute, Shenzhen, China.
² Food Safety and Technology Research Center, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong; Shenzhen Key Laboratory for Food Biological Safety Control, PolyU Shenzhen Research Institute, Shenzhen, China. Electronic address: kahing.wong@polyu.edu.hk.
³ Food Safety and Technology Research Center, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong; Shenzhen Key Laboratory for Food Biological Safety Control, PolyU Shenzhen Research Institute, Shenzhen, China. Electronic address: samuel.chun-lap.lo@polyu.edu.hk.

PMID: 29544834
DOI: 10.1016/j.foodres.2016.04.002

Abstract

For centuries, edible bird's nest (EBN) has been consumed as a Chinese delicacy. In the past decades, numerous studies reported that water soluble extract of the EBN not only possessed epidermal growth factor, but also associated with a wide range of health-promoting effects. However, based on the traditional Chinese way of EBN preparation and consumption, the bioactive components should be originated from both its hot water soluble and insoluble fractions. Nevertheless, information on the hot water insoluble fraction (HWIF) of EBN is not currently available. In this study, peptides released from the HWIF of EBN under simulated gastro-intestinal conditions were identified for the first time by de novo sequencing using a combination of MALDI TOF/TOF MS and ESI-ion-trap MS/MS. The released peptides were found to share very high similarities to mucin, NADH dehydrogenase, acidic mammalian chitinase-like protein, immunoglobulin, proline-rich protein, von Willebrand factor and epidermal growth factor domain-containing protein.

Keywords: Edible bird's nest; Hot water insoluble fraction; Peptides; Simulated gastro-intestinal conditions.