Inter-method reproducibility of biexponential R2 MR relaxometry for estimation of liver iron concentration

Abstract

Purpose: To assess the reproducibility of biexponential R₂ -relaxometry MRI for estimation of liver iron concentration (LIC) between proprietary and nonproprietary analysis methods.

Methods: This single-center retrospective study, approved by investigational review board and compliant with the Health Insurance Portability and Accountability Act, included 40 liver MRI exams in 38 subjects with suspected or known iron overload. From spin-echo images of the liver, acquired at 5 different echo times (TE = 6-18 ms), biexponential R₂ maps were calculated using 1 proprietary (FerriScan, Resonance Health Ltd., Claremont WA, Australia) and 3 nonproprietary (simulated annealing, nonlinear least squares, dictionary search) analysis methods. Each subject's average liver R₂ value was converted to LIC using a previously validated calibration curve. Inter-method reproducibility for liver R₂ and LIC were assessed for linearity using linear regression analysis and absolute agreement using intraclass correlation and Bland-Altman analysis. For point estimates, 95% confidence intervals were calculated; P values < 0.05 were considered statistically significant.

Results: Linearity between the proprietary and nonproprietary methods was excellent across the observed range for R₂ (20-312 s^-1 ) and LIC (0.4-52.2 mg/g), with all coefficients of determination (R² ) ≥ 0.95. No statistically significant bias was found (slope estimates ∼ 1; intercept estimates ∼ 0; P values > 0.05). Agreement between the 4 methods was excellent for both liver R₂ and LIC (intraclass correlations ≥ 0.97). Bland-Altman 95% limits of agreement in % difference between the proprietary and nonproprietary methods were ≤ 9% and ≤ 16% for R₂ and LIC, respectively.

Conclusion: Biexponential R₂ -relaxometry MRI for LIC estimation is reproducible between proprietary and nonproprietary analysis methods.

Keywords: R2 relaxation; T2 relaxation; hemochromatosis; hemosiderosis; iron overload; liver iron concentration; reproducibility.