The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion

J Cell Biol. 2018 Jul 2;217(7):2445-2462. doi: 10.1083/jcb.201709009. Epub 2018 Jun 6.

Abstract

Ploidy is tightly regulated in eukaryotic cells and is critical for cell function and survival. Cells coordinate multiple pathways to ensure replicated DNA is segregated accurately to prevent abnormal changes in chromosome number. In this study, we characterize an unanticipated role for the Saccharomyces cerevisiae "remodels the structure of chromatin" (RSC) complex in ploidy maintenance. We show that deletion of any of six nonessential RSC genes causes a rapid transition from haploid to diploid DNA content because of nondisjunction events. Diploidization is accompanied by diagnostic changes in cell morphology and is stably maintained without further ploidy increases. We find that RSC promotes chromosome segregation by facilitating spindle pole body (SPB) duplication. More specifically, RSC plays a role in distributing two SPB insertion factors, Nbp1 and Ndc1, to the new SPB. Thus, we provide insight into a role for a SWI/SNF family complex in SPB duplication and ploidy maintenance.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / genetics*
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosome Segregation / genetics
  • Cytoskeletal Proteins / genetics*
  • DNA-Binding Proteins / genetics*
  • Nuclear Envelope / genetics
  • Nuclear Pore Complex Proteins / genetics*
  • Nuclear Proteins / genetics*
  • Ploidies
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins / genetics*
  • Spindle Apparatus / genetics
  • Spindle Pole Bodies / genetics*
  • Transcription Factors / genetics*

Substances

  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • Cytoskeletal Proteins
  • DNA-Binding Proteins
  • NBP1 protein, S cerevisiae
  • NDC1 protein, S cerevisiae
  • Nuclear Pore Complex Proteins
  • Nuclear Proteins
  • RSC complex, S cerevisiae
  • SWI-SNF-B chromatin-remodeling complex
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors