Intravenous fish oil containing lipid emulsion attenuates inflammatory cytokines and the development of bronchopulmonary dysplasia in very premature infants: A double-blind, randomized controlled trial

Clin Nutr. 2019 Jun;38(3):1045-1052. doi: 10.1016/j.clnu.2018.06.929. Epub 2018 Jun 18.

Abstract

Background & aims: Preterm infants have lower levels of long-chain polyunsaturated fatty acids (LCPUFAs). Supplementing very premature infants with intravenous lipid emulsions that fish oil, which is rich in n-3 LC-PUFAs, may decrease bronchopulmonary dysplasia (BPD) by modulating inflammation and neonatal immune function.

Methods: Sixty very low birth weight (VLBW) premature infants requiring ventilator support were randomized in a double-blind manner to 2 groups and received total parenteral nutrition with fish oil containing LE (intervention group, n = 30) or soybean oil containing LE (control group, n = 30) for 7 days. Blood samples and bronchoalveolar lavage fluid (BALF) were obtained for assay on day 1 and 7 days after LE. The primary outcome was to compare the levels of interleukin (IL)-1β and IL-6 in serum and BALF. Secondary outcomes were to compare mortality and co-morbidities.

Results: The levels of IL-1β and IL-6 in serum and BALF were significantly lower in the intervention group at day 8 (p < 0.05). The incidence of BPD in the intervention group compared to the control group was 13.3% versus 36.7% (p = 0.04; odds ratio [OR], 0.36; 95% confidence interval [CI], 0.21-0.86). The duration of ventilator support and oxygen use was significantly less in the intervention group than in the control group (p < 0.05). The level of alanine aminotransferase was significantly lower in the intervention group on day 8 (p = 0.031).

Conclusions: In very premature infants, early administration of fish oil containing LE significantly decreased IL-1β and IL-6 levels in serum and BALF and was associated with shorter duration of ventilator support and less bronchopulmonary dysplasia (BPD).

Trial registration number: ISRCTN 11427103.

Keywords: Bronchopulmonary dysplasia; DHA; Fish oil; Premature infants; n-3 long-chain PUFA.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intravenous
  • Bronchoalveolar Lavage Fluid / chemistry
  • Bronchopulmonary Dysplasia* / epidemiology
  • Bronchopulmonary Dysplasia* / prevention & control
  • Bronchopulmonary Dysplasia* / therapy
  • Comorbidity
  • Cytokines / analysis*
  • Double-Blind Method
  • Fat Emulsions, Intravenous* / administration & dosage
  • Fat Emulsions, Intravenous* / therapeutic use
  • Fatty Acids, Omega-3 / administration & dosage
  • Fatty Acids, Omega-3 / therapeutic use
  • Female
  • Fish Oils* / administration & dosage
  • Fish Oils* / therapeutic use
  • Humans
  • Infant, Newborn
  • Infant, Premature
  • Infant, Very Low Birth Weight
  • Male

Substances

  • Cytokines
  • Fat Emulsions, Intravenous
  • Fatty Acids, Omega-3
  • Fish Oils

Associated data

  • ISRCTN/ISRCTN11427103