Abstract
A 'rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human beta-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Chromosome Mapping
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Cloning, Molecular
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DNA Restriction Enzymes
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DNA Transposable Elements*
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DNA, Recombinant / metabolism*
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Genes*
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Globins / genetics*
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Humans
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Nucleic Acid Hybridization
Substances
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DNA Transposable Elements
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DNA, Recombinant
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Globins
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DNA Restriction Enzymes