Characterising skeletal muscle haemoglobin saturation during exercise using near-infrared spectroscopy in chronic kidney disease

Clin Exp Nephrol. 2019 Jan;23(1):32-42. doi: 10.1007/s10157-018-1612-0. Epub 2018 Jun 30.

Abstract

Background: Chronic kidney disease (CKD) patients have reduced exercise capacity. Possible contributing factors may include impaired muscle O2 utilisation through reduced mitochondria number and/or function slowing the restoration of muscle ATP concentrations via oxidative phosphorylation. Using near-infrared spectroscopy (NIRS), we explored changes in skeletal muscle haemoglobin/myoglobin O2 saturation (SMO2%) during exercise.

Methods: 24 CKD patients [58.3 (± 16.5) years, eGFR 56.4 (± 22.3) ml/min/1.73 m2] completed the incremental shuttle walk test (ISWT) as a marker of exercise capacity. Using NIRS, SMO2% was measured continuously before, during, and after (recovery) exercise. Exploratory differences were investigated between exercise capacity tertiles in CKD, and compared with six healthy controls.

Results: We identified two discrete phases; a decline in SMO2% during incremental exercise, followed by rapid increase upon cessation (recovery). Compared to patients with low exercise capacity [distance walked during ISWT, 269.0 (± 35.9) m], patients with a higher exercise capacity [727.1 (± 38.1) m] took 45% longer to reach their minimum SMO2% (P = .038) and recovered (half-time recovery) 79% faster (P = .046). Compared to controls, CKD patients took significantly 56% longer to recover (i.e., restore SMO2% to baseline, full recovery) (P = .014).

Conclusions: Using NIRS, we have determined for the first time in CKD, that favourable SMO2% kinetics (slower deoxygenation rate, quicker recovery) are associated with greater exercise capacity. These dysfunctional kinetics may indicate reduced mitochondria capacity to perform oxidative phosphorylation-a process essential for carrying out even simple activities of daily living. Accordingly, NIRS may provide a simple, low cost, and non-invasive means to evaluate muscle O2 kinetics in CKD.

Keywords: Chronic kidney disease; Exercise; Haemoglobin; Near-infrared spectroscopy; Oxygen saturation.

MeSH terms

  • Adult
  • Aged
  • Anaerobic Threshold
  • Diabetes Mellitus, Type 2 / complications
  • Diabetes Mellitus, Type 2 / physiopathology
  • Exercise Test
  • Exercise*
  • Female
  • Glomerular Filtration Rate
  • Healthy Volunteers
  • Hemoglobins / metabolism*
  • Humans
  • Male
  • Middle Aged
  • Muscle, Skeletal / metabolism*
  • Myoglobin / analysis
  • Myoglobin / metabolism
  • Oxygen Consumption
  • Renal Insufficiency, Chronic / metabolism*
  • Spectroscopy, Near-Infrared

Substances

  • Hemoglobins
  • Myoglobin