Enhancing T cell therapy through TCR-signaling-responsive nanoparticle drug delivery

Nat Biotechnol. 2018 Sep;36(8):707-716. doi: 10.1038/nbt.4181. Epub 2018 Jul 9.

Abstract

Adoptive cell therapy (ACT) with antigen-specific T cells has shown remarkable clinical success; however, approaches to safely and effectively augment T cell function, especially in solid tumors, remain of great interest. Here we describe a strategy to 'backpack' large quantities of supporting protein drugs on T cells by using protein nanogels (NGs) that selectively release these cargos in response to T cell receptor activation. We designed cell surface-conjugated NGs that responded to an increase in T cell surface reduction potential after antigen recognition and limited drug release to sites of antigen encounter, such as the tumor microenvironment. By using NGs that carried an interleukin-15 super-agonist complex, we demonstrated that, relative to systemic administration of free cytokines, NG delivery selectively expanded T cells 16-fold in tumors and allowed at least eightfold higher doses of cytokine to be administered without toxicity. The improved therapeutic window enabled substantially increased tumor clearance by mouse T cell and human chimeric antigen receptor (CAR)-T cell therapy in vivo.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation
  • Cytokines / administration & dosage
  • Drug Delivery Systems*
  • Humans
  • Immunotherapy, Adoptive*
  • Interleukin-15 / administration & dosage
  • Leukocyte Common Antigens / immunology
  • Lymphocyte Activation
  • Mice
  • Nanoparticles*
  • Receptors, Antigen, T-Cell / metabolism*
  • Signal Transduction*
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • Tumor Microenvironment

Substances

  • Cytokines
  • IL15 protein, human
  • Interleukin-15
  • Receptors, Antigen, T-Cell
  • Leukocyte Common Antigens