Mosaicism due to postzygotic mutations in women with focal dermal hypoplasia

Br J Dermatol. 2019 Mar;180(3):657-661. doi: 10.1111/bjd.17024. Epub 2018 Sep 30.

Abstract

Focal dermal hypoplasia (FDH, Goltz syndrome, MIM #305600) constitutes a rare multisystem genetic disorder of the skin, skeleton, teeth and eyes with considerable variation in the clinical features. FDH is transmitted as an X-linked dominant trait and is caused by mutations in PORCN. In male children, hemizygous PORCN mutations are lethal in utero. Around 300 cases have been reported in the literature to date. About 10% of them are male patients presenting with either Klinefelter syndrome (karyotype 47, XXY) or mosaicism of a postzygotic mutation. Here we describe four cases of women with typical features of FDH, in whom a PORCN mutation was found in DNA from affected cutaneous tissue but not in DNA from peripheral blood. This study suggests that mosaicism caused by a postzygotic mutation occurs more often than assumed to date in female patients with FDH. A negative analysis performed on peripheral blood DNA does not exclude the diagnosis of FDH and it is therefore of practical importance to analyse DNA from the affected skin in order to identify low-level mosaicism and thus to improve diagnostic precision. In total, we found two missense variants, one novel indel and one novel splice-site variant. Individuals harbouring postzygotic mosaicism run a risk of transmitting the disorder to their daughters, because the maternal mosaic could also affect the gonads.

Publication types

  • Case Reports

MeSH terms

  • Acyltransferases / genetics*
  • Adult
  • DNA Mutational Analysis
  • Female
  • Focal Dermal Hypoplasia / blood
  • Focal Dermal Hypoplasia / genetics*
  • Focal Dermal Hypoplasia / pathology
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Membrane Proteins / genetics*
  • Mosaicism*
  • Mouth Mucosa / pathology
  • Skin / pathology
  • Young Adult
  • Zygote

Substances

  • Membrane Proteins
  • Acyltransferases
  • PORCN protein, human