A Structural Basis for 129 Xe Hyper-CEST Signal in TEM-1 β-Lactamase

Chemphyschem. 2019 Jan 21;20(2):260-267. doi: 10.1002/cphc.201800624. Epub 2018 Sep 13.

Abstract

Genetically encoded (GE) contrast agents detectable by magnetic resonance imaging (MRI) enable non-invasive visualization of gene expression and cell proliferation at virtually unlimited penetration depths. Using hyperpolarized 129 Xe in combination with chemical exchange saturation transfer, an MR contrast approach known as hyper-CEST, enables ultrasensitive protein detection and biomolecular imaging. GE MRI contrast agents developed to date include nanoscale proteinaceous gas vesicles as well as the monomeric bacterial proteins TEM-1 β-lactamase (bla) and maltose binding protein (MBP). To improve understanding of hyper-CEST NMR with proteins, structural and computational studies were performed to further characterize the Xe-bla interaction. X-ray crystallography validated the location of a high-occupancy Xe binding site predicted by MD simulations, and mutagenesis experiments confirmed this Xe site as the origin of the observed CEST contrast. Structural studies and MD simulations with representative bla mutants offered additional insight regarding the relationship between local protein structure and CEST contrast.

Keywords: CEST; Xenon; contrast; hyperpolarized; magnetic resonance.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Allosteric Site
  • Binding Sites
  • Contrast Media / chemistry
  • Crystallography, X-Ray
  • Limit of Detection
  • Maltose-Binding Proteins / chemistry
  • Nuclear Magnetic Resonance, Biomolecular
  • Protein Conformation
  • Xenon Isotopes / chemistry*
  • beta-Lactamases / chemistry*

Substances

  • Contrast Media
  • Maltose-Binding Proteins
  • Xenon Isotopes
  • beta-Lactamases
  • beta-lactamase TEM-1