Clinical implementation of the sFlt-1/PlGF ratio to identify preeclampsia and fetal growth restriction: A prospective cohort study

Pregnancy Hypertens. 2018 Jul:13:279-285. doi: 10.1016/j.preghy.2018.06.017. Epub 2018 Jun 30.

Abstract

Objective: To analyze the usefulness of a clinical protocol for early detection of preeclampsia and/or fetal growth restriction (PE/FGR) using, in previously selected pregnancies, the measurement of the sFlt-1/PlGF ratio at 24-28 weeks of gestation.

Study design: Prospective observational cohort study carried out in a single tertiary hospital in Spain. 5601 consecutive singleton pregnancies with complete follow-up were included. High-risk women for PE/FGR were selected by combining data from maternal history and second trimester uterine artery Doppler. Subsequently these patients underwent intensive monitoring, including the measurement of the sFlt-1/PlGF ratio at 24-28 weeks to predict PE/FGR.

Main outcome measures: Early, intermediate and late PE/FGR (delivery <32 + 0, 32 + 0 - <36 + 0 and ≥36 + 0 weeks, respectively).

Results: Overall incidence of early, intermediate and late PE/FGR was 0.3%, 0.7% and 3.2%, respectively, being higher in the 4.3% of women selected for intensive monitoring: 5.8%, 8.7% and 15.4%, respectively (all p < 0.001). The area under the curve (AUC) with 95%CI of the sFlt-1/PlGF ratio for detecting early PE/FGR was 0.98 (0.97-1.00), and the sFlt-1/PlGF ratio >95th centile showed a sensitivity (%) of 100 (95%CI, 78.5-100) and specificity (%) of 80.6 (95%CI, 75.0-85.2). The AUC of the sFlt-1/PlGF ratio for detecting intermediate and late PE/FGR was of 0.87 (95%CI, 0.77-0.97) and 0.68 (95%CI, 0.58-0.79), respectively.

Conclusion: A contingent strategy of measuring the sFlt-1/PlGF ratio at 24-28 weeks in women previously selected by clinical factors and uterine artery Doppler enables an accurate prediction of PE/FGR. This performance is optimal to predict PE/FGR requiring delivery before 32 weeks.

Keywords: Fetal growth restriction; PlGF; Preeclampsia; sFlt-1.

Publication types

  • Observational Study

MeSH terms

  • Adult
  • Biomarkers / blood
  • Blood Pressure
  • Early Diagnosis
  • Female
  • Fetal Growth Retardation / blood*
  • Fetal Growth Retardation / diagnosis
  • Fetal Growth Retardation / physiopathology
  • Fetal Weight
  • Humans
  • Placenta Growth Factor / blood*
  • Pre-Eclampsia / blood*
  • Pre-Eclampsia / diagnosis
  • Pre-Eclampsia / physiopathology
  • Predictive Value of Tests
  • Pregnancy
  • Pregnancy Outcome
  • Prognosis
  • Prospective Studies
  • Risk Factors
  • Spain
  • Ultrasonography, Doppler
  • Ultrasonography, Prenatal / methods
  • Uterine Artery / diagnostic imaging
  • Uterine Artery / physiopathology
  • Vascular Endothelial Growth Factor Receptor-1 / blood*

Substances

  • Biomarkers
  • PGF protein, human
  • Placenta Growth Factor
  • FLT1 protein, human
  • Vascular Endothelial Growth Factor Receptor-1