Oncogenic human papillomavirus infection and genotypes characterization among sexually active women in Tenkodogo at Burkina Faso, West Africa

Papillomavirus Res. 2018 Dec:6:22-26. doi: 10.1016/j.pvr.2018.09.001. Epub 2018 Sep 19.

Abstract

Objective: This study was conducted to determine the prevalence and distribution of high-risk human papillomavirus (HR-HPV) genotypes among sexually active women in Tenkodogo, Burkina Faso.

Methods: Among 131 sexually active women attending the Tenkodogo Urban Medical Center, endocervical samples were collected prior to screening for precancerous lesions. After viral DNA extraction, fourteen HR-HPV genotypes were characterized by real-time multiplex PCR in these cervical samples.

Results: The mean age was 35.5 ± 9.5 years. Of the 131 women, 45 were infected with at least one HR-HPV genotype. The prevalence of HR-HPV infection among these women was 34.4%. Among the 45 oncogenic HPV-infected women, single HR-HPV genotype was found in 55.6% while 44.4% were infected with more than one HR-HPV genotype. The most frequent genotypes were HPV56 (36.5%), HPV66 (36.5%).

Conclusion: Tenkodogo women included in this study had a higher prevalence of HPV 56, HPV 66. A larger study with a more representative sample would therefore be needed to determine predominant oncogenic genotypes in the subregion and especially in cancer cases.

Keywords: Genotypes; High-risk HPV; Multiple infections; Real time PCR; Tenkodogo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Burkina Faso / epidemiology
  • Cervix Uteri / virology
  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Female
  • Genotype*
  • Genotyping Techniques
  • Humans
  • Middle Aged
  • Multiplex Polymerase Chain Reaction
  • Papillomaviridae / classification*
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / epidemiology*
  • Papillomavirus Infections / virology*
  • Prevalence
  • Real-Time Polymerase Chain Reaction
  • Young Adult

Substances

  • DNA, Viral