Positive correlation between proteasome activity and polyphenols in the telencephalon of adult female mice

Rev Fac Cien Med Univ Nac Cordoba. 2018 Sep 25;75(3):189-193. doi: 10.31053/1853.0605.v75.n3.19930.

Abstract

Introduction: Proteasome regulates proteostasis, and can be compromised in neurodegenerative diseases. Thus, our aim was to correlate the activity of proteasome to the level of polyphenols in telencephalon during murine adulthood.

Methods: Proteasome activity, polyphenols and other variables (glucose and hydroperoxides) were analysed in Balb/c female telencephala (n = 20, age = 4-12 months), using multivariate methods.

Results: The following values were found: proteasome activity = 3.1 ± 0.6 FI/µg of tissue proteins, glucose = 0.1 ± 0.0 µg/µg, hydroperoxides = 363.4 ± 96.6 OD/µg, and polyphenols = 0.1 ± 0.0 ng/µg. Polyphenols reduced during aging showed a direct correlation with proteasome (Pearson's coefficient = 0.43, p = 0.0590, and a multivariate linear regressive coefficient = 17.85, p = 0.0216), with glucose and hydroperoxides being not involved (p>0.1). This correlation was confirmed by partial least square regression (beta = 0.67).

Conclusion: Proteasome activity can be affected during ageing, and promoted by telencephalic polyphenol levels. Thus, these diet compounds might exert benefits in adult brain.

Keywords: central nervous system; proteostasis; phytochemicals; multivariate statistics.

MeSH terms

  • Animals
  • Body Weight
  • Female
  • Glucose / analysis*
  • Glucose / metabolism
  • Hydrogen Peroxide / analysis*
  • Hydrogen Peroxide / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Polyphenols / analysis*
  • Polyphenols / metabolism
  • Proteasome Endopeptidase Complex / analysis*
  • Proteasome Endopeptidase Complex / metabolism
  • Telencephalon / chemistry*

Substances

  • Polyphenols
  • Hydrogen Peroxide
  • Proteasome Endopeptidase Complex
  • Glucose