Differences in the Epstein-Barr Virus gp350 IgA Antibody Response Are Associated With Increased Risk for Coinfection With a Second Strain of Epstein-Barr Virus

J Infect Dis. 2019 Feb 23;219(6):955-963. doi: 10.1093/infdis/jiy601.

Abstract

Background: The Epstein-Barr virus (EBV) viral glycoprotein gp350 has been proposed as a candidate antigen for an EBV vaccine. However, the proposed formulations of these vaccines have not taken into account the presence of 2 unique EBV strains (EBV-1 and EBV-2) present in areas of high incidence of the EBV-associated cancer, Burkitt lymphoma.

Methods: In this study, we analyze the kinetics of EBV-1 and EBV-2 infection in an asymptomatic infant cohort from Kisumu, Kenya. We also analyzed the kinetics of the antibody response against 5 EBV antigens, gp350 (IgG and IgA), VCA (IgG), EBNA-1 (IgG), EAd (IgG), and Zta (IgG).

Results: We observed a high frequency of coinfection with both EBV types over time, with the only observable defect in the antibody response in infants coinfected being a significantly lower level of anti-gp350 IgA at peak response. Gp350 IgA levels were also significantly lower in coinfected infants 2.5 months postinfection and at the time of coinfection.

Conclusions: These results suggest that anti-gp350 IgA antibodies may be important for sterilizing immunity against secondary infection. These findings have implications for the development of an efficacious EBV vaccine to prevent both EBV-1 and EBV-2 infection in a population at high risk for Burkitt lymphoma.

Keywords: Epstein-Barr virus; antibody; primary infection.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Aged
  • Antibodies, Viral / immunology*
  • Child
  • Child, Preschool
  • Coinfection / immunology
  • Epstein-Barr Virus Infections / classification
  • Epstein-Barr Virus Infections / immunology*
  • Epstein-Barr Virus Nuclear Antigens / immunology
  • Female
  • Herpesvirus 4, Human / immunology*
  • Humans
  • Immunoglobulin A / immunology*
  • Infant
  • Kenya
  • Male
  • Membrane Glycoproteins / immunology*
  • Middle Aged
  • Viral Load

Substances

  • Antibodies, Viral
  • Epstein-Barr Virus Nuclear Antigens
  • Immunoglobulin A
  • Membrane Glycoproteins