In vitro activity of tedizolid against clinical isolates of Staphylococcus lugdunensis and Staphylococcus haemolyticus from Europe and the United States

Diagn Microbiol Infect Dis. 2019 Jan;93(1):85-88. doi: 10.1016/j.diagmicrobio.2018.08.006. Epub 2018 Aug 23.

Abstract

Staphylococcus lugdunensis and Staphylococcus haemolyticus are unique among CoNS in that the former often causes aggressive disease, while the latter consistently exhibits high rates of multidrug resistance. We evaluated the in vitro susceptibility of contemporary (2012-2013) isolates from both pathogens to tedizolid and comparators, using standard methodology. Results were interpreted using CLSI and EUCAST breakpoints. Overall, 106 S. lugdunensis and 103 S. haemolyticus isolates were collected from 51 medical centers in the United States and 30 centers in 18 European countries. Tedizolid showed good activity against S. lugdunensis (MIC50/MIC90: 0.12/0.12 mg/L) and S. haemolyticus (MIC50/MIC90: 0.12/0.12 mg/L), inhibiting all isolates at MIC ≤0.25 mg/L. Based on the EUCAST breakpoint for staphylococci and when substituting the CLSI breakpoint for Staphylococcus aureus, all isolates were tedizolid susceptible. All isolates were also susceptible to linezolid, but the in vitro potency of tedizolid was 4-fold greater than that of linezolid against both S. lugdunensis and S. haemolyticus, based on MIC90 values. S. lugdunensis exhibited ≥99% susceptibility to vancomycin, teicoplanin, gentamicin, levofloxacin, and trimethoprim-sulfamethoxazole; 7% of isolates were resistant to tetracycline, 11% to clindamycin, and 2% were methicillin-resistant. S. haemolyticus exhibited high rates of resistance to commonly used anti-staphylococcal agents: 71% of isolates were resistant to methicillin, 36%-37% to clindamycin, and 30%-50% to gentamicin. These in vitro findings suggest that tedizolid could be an alternative treatment option for infections due to these medically important CoNS pathogens. Additional clinical evaluation and continued surveillance of tedizolid in vitro activity against S. lugdunensis and S. haemolyticus are warranted.

Keywords: Coagulase-negative staphylococci; Gram-positive; Oxazolidinone; Susceptibility.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Coagulase / deficiency
  • Drug Resistance, Bacterial / drug effects
  • Humans
  • Microbial Sensitivity Tests / standards
  • Microbial Viability / drug effects
  • Oxazolidinones / pharmacology*
  • Staphylococcal Infections / microbiology
  • Staphylococcus haemolyticus / drug effects*
  • Staphylococcus haemolyticus / isolation & purification
  • Staphylococcus lugdunensis / drug effects*
  • Staphylococcus lugdunensis / isolation & purification
  • Tetrazoles / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Coagulase
  • Oxazolidinones
  • Tetrazoles
  • tedizolid