Analysis of drug resistance in HIV protease

BMC Bioinformatics. 2018 Oct 22;19(Suppl 11):362. doi: 10.1186/s12859-018-2331-y.

Abstract

Background: Drug resistance in HIV is the major problem limiting effective antiviral therapy. Computational techniques for predicting drug resistance profiles from genomic data can accelerate the appropriate choice of therapy. These techniques can also be used to select protease mutants for experimental studies of resistance and thereby assist in the development of next-generation therapies.

Results: The machine learning produced highly accurate and robust classification of HIV protease resistance. Genotype data were mapped to the enzyme structure and encoded using Delaunay triangulation. Generative machine learning models trained on one inhibitor could classify resistance from other inhibitors with varying levels of accuracy. Generally, the accuracy was best when the inhibitors were chemically similar.

Conclusions: Restricted Boltzmann Machines are an effective machine learning tool for classification of genomic and structural data. They can also be used to compare resistance profiles of different protease inhibitors.

Keywords: Drug resistance; HIV protease; Machine learning; RBM; Structure-based.

MeSH terms

  • Algorithms
  • Databases as Topic
  • Drug Resistance, Viral / drug effects
  • Drug Resistance, Viral / genetics*
  • Genotype
  • HIV Infections / drug therapy
  • HIV Protease / genetics*
  • HIV Protease Inhibitors / chemistry
  • HIV Protease Inhibitors / pharmacology
  • Humans
  • Machine Learning
  • Principal Component Analysis

Substances

  • HIV Protease Inhibitors
  • HIV Protease