Neural stem cell-derived factors inhibit the growth and invasion of U87 stem-like cells in vitro

J Cell Biochem. 2019 Apr;120(4):5472-5479. doi: 10.1002/jcb.27826. Epub 2018 Oct 26.

Abstract

Glioma is one of the most common and aggressive tumors in the brain. Significant attention has been paid to the potential use of neural stem/progenitor cells (NSCs/NPCs) as delivery vehicles to cure gliomas. However, whether the NSCs/NPCs or the factors they produced could make a contribution still remains to be seen. In this study, we focused on the inhibitory effects of the factors produced by NSCs/NPCs on the biological behavior of the glioma stem-like cell in vitro. The human glioma cell line U87 was selected and the U87 stem-like cells were addressed. After being cultured in the NSC condition medium (NSC-CM), the viability and proliferation of U87 stem-like cells were significantly reduced. The invasion of U87 stem-like cells and the migration of U87 cells were also significantly decreased. However, no significant change was observed in regard to the astrocytic differentiation of U87 stem-like cells. These indicated that NSCs/NPCs produced some factors and had an inhibitory effect on the growth and invasion but not the terminal differentiation of U87 stem-like cells. It is worth paying attention to NSCs/NPCs as a high-potential candidate for glioma treatment.

Keywords: U87 stem-like cell; condition medium; invasion; neural stem/progenitor cells; survival.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Glioma / metabolism*
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Neoplasm Invasiveness
  • Neoplastic Stem Cells / metabolism*
  • Neural Stem Cells / metabolism*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Intercellular Signaling Peptides and Proteins