Therapeutic Efficacy of Melatonin Against Polycystic Ovary Syndrome (PCOS) Induced by Letrozole in Wistar Rat

Pak J Biol Sci. 2018 Jan;21(7):340-347. doi: 10.3923/pjbs.2018.340.347.

Abstract

Background and objectives: Polycystic ovary syndrome (PCOS) is a common heterogeneous endocrinological and metabolic disorder in women of reproductive age which leads to infertility/subfertility. The present study was commenced to elucidate the therapeutic efficacy of melatonin in the pathogenesis of letrozole induced polycystic ovary syndrome (PCOS) in Wistar rat.

Materials and methods: Letrozole was administered orally (1 mg kg-1) to induce PCOS condition in Wistar female rats for a period of 2-3 weeks followed by a dose of melatonin (200 µg/100 g b.wt.) to PCOS induced rats. On the completion of experimental period the level of cytokines and expression level of different receptors was assessed.

Results: The PCOs rats showed down regulation in melatonin (MT1 and MT2), estrogen (ER-α) and cytokine (IL-2R and IL-6R) receptors expression and high circulatory level of IL-6 and TNF-α during PCO condition. These anomalies in expression pattern and circulatory level of cytokines were restored following the treatment.

Conclusion: Finding of present study showed the role of melatonin supplementation at receptor level and also suggesting a crosstalk between MT1R / MT2R via cytokine IL-2R and IL-6R resulting in modulation of ER-α receptors.

Keywords: Melatonin; endocrinological; estrogen receptor; letrozole; melatonin receptors; metabolic disorder; polycystic ovary syndrome.

MeSH terms

  • Administration, Oral
  • Animals
  • Disease Models, Animal
  • Estrogens / metabolism
  • Female
  • Letrozole / pharmacology*
  • Melatonin / pharmacology*
  • Polycystic Ovary Syndrome / chemically induced*
  • Polycystic Ovary Syndrome / drug therapy*
  • Polycystic Ovary Syndrome / metabolism
  • Rats
  • Rats, Wistar
  • Receptors, Interleukin-2 / metabolism
  • Receptors, Interleukin-6 / metabolism
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Estrogens
  • Receptors, Interleukin-2
  • Receptors, Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Letrozole
  • Melatonin