Plant HP1 protein ADCP1 links multivalent H3K9 methylation readout to heterochromatin formation

Cell Res. 2019 Jan;29(1):54-66. doi: 10.1038/s41422-018-0104-9. Epub 2018 Nov 13.

Abstract

Heterochromatin Protein 1 (HP1) recognizes histone H3 lysine 9 methylation (H3K9me) through its conserved chromodomain and maintains heterochromatin from fission yeast to mammals. However, in Arabidopsis, Like Heterochromatin Protein 1 (LHP1) recognizes and colocalizes genome-wide with H3K27me3, and is the functional homolog of Polycomb protein. This raises the question whether genuine HP1 homologs exist in plants. Here, we report on the discovery of ADCP1, a plant-specific triple tandem Agenet protein, as a multivalent H3K9me reader in Arabidopsis, and establish that ADCP1 is essential for heterochromatin formation and transposon silencing through modulating H3K9 and DNA methylation levels. Structural studies revealed the molecular basis underlying H3K9me-specific recognition by tandem Agenet of ADCP1. Similar to human HP1α and fly HP1a, ADCP1 mediates heterochromatin phase separation. Our results demonstrate that despite its distinct domain compositions, ADCP1 convergently evolves as an HP1-equivalent protein in plants to regulate heterochromatin formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / physiology*
  • Chromatin Assembly and Disassembly
  • Chromobox Protein Homolog 5
  • Chromosomal Proteins, Non-Histone / physiology*
  • Cloning, Molecular
  • DNA Methylation
  • Escherichia coli / genetics
  • Heterochromatin / metabolism*
  • Histones / metabolism*
  • Transcription Factors / physiology*

Substances

  • Arabidopsis Proteins
  • At1g09320 protein, Arabidopsis
  • CBX5 protein, human
  • Chromosomal Proteins, Non-Histone
  • Heterochromatin
  • Histones
  • LHP1 protein, Arabidopsis
  • Transcription Factors
  • Chromobox Protein Homolog 5