Lipase mediated functionalization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with ascorbic acid into an antioxidant active biomaterial

Int J Biol Macromol. 2019 Feb 15:123:117-123. doi: 10.1016/j.ijbiomac.2018.11.052. Epub 2018 Nov 11.

Abstract

Naturally produced polyhydroxyalkanoates (PHAs) biopolymers have limited medical applications due to their brittle and hydrophobic nature. In this study poly(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV) copolymer was produced using engineered Escherichia coli YJ101, and further functionalized with ascorbic acid using Candida antarctica lipase B mediated esterification. Copolymer P(3HB-co-3HV)-ascorbic acid showed lower degree of crystallinity (9.96%), higher thermal degradation temperature (294.97 °C) and hydrophilicity (68°) as compared to P(3HB-co-3HV). Further, P(3HB-co-3HV)-ascorbic acid biomaterial showed 14% scavenging effect on 1,1-diphenyl-2-picryl-hydrazyl (DPPH), and 1.6 fold increase in biodegradability as compared to P(3HB-co-3HV). Improvement of PHAs polymer properties by adding functional groups could be a good approach to increase their biodegradability, economic value and important applications in the medical field.

Keywords: Antioxidant; Biomaterial; Copolymer; Esterification; Polyhydroxyalkanoates.

MeSH terms

  • Antioxidants / metabolism*
  • Ascorbic Acid / metabolism*
  • Biocompatible Materials / metabolism*
  • Biodegradation, Environmental
  • Biphenyl Compounds / metabolism
  • Escherichia coli / metabolism
  • Fungal Proteins / metabolism*
  • Lipase / metabolism*
  • Picrates / metabolism
  • Polyesters / metabolism*
  • Polyhydroxyalkanoates / metabolism
  • Polymers / metabolism

Substances

  • Antioxidants
  • Biocompatible Materials
  • Biphenyl Compounds
  • Fungal Proteins
  • Picrates
  • Polyesters
  • Polyhydroxyalkanoates
  • Polymers
  • poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate)
  • 1,1-diphenyl-2-picrylhydrazyl
  • Lipase
  • lipase B, Candida antarctica
  • Ascorbic Acid