Isolation and partial characterisation of a 26 kilodalton antigen from Plasmodium falciparum recognised by an inhibitory monoclonal antibody

R Ramasamy; R J Simpson; A Dexter; M Keeghan; C Reed; G Bushell; L T Ingram; T Henderson; M B Moloney; R L Moritz

doi:10.1016/0166-6851(88)90067-9

Isolation and partial characterisation of a 26 kilodalton antigen from Plasmodium falciparum recognised by an inhibitory monoclonal antibody

Mol Biochem Parasitol. 1988 Jun;29(2-3):125-32. doi: 10.1016/0166-6851(88)90067-9.

Authors

R Ramasamy¹, R J Simpson, A Dexter, M Keeghan, C Reed, G Bushell, L T Ingram, T Henderson, M B Moloney, R L Moritz, et al.

Affiliation

¹ Queensland Institute of Medical Research, Brisbane, Australia.

PMID: 3045539
DOI: 10.1016/0166-6851(88)90067-9

Abstract

A 26 kDa protein, present in trophozoites and schizonts of Plasmodium falciparum, has been identified as the target of a monoclonal antibody that weakly inhibits parasite growth in vitro. The antigen has been purified to homogeneity by immuno-affinity chromatography and electrophoresis. The sequence of 19 amino acids at the N-terminus of the protein has been determined.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Monoclonal / immunology*
Antigens, Protozoan / analysis
Antigens, Protozoan / immunology
Antigens, Protozoan / isolation & purification*
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique
Immunoassay
Molecular Sequence Data
Plasmodium falciparum / growth & development
Plasmodium falciparum / immunology*

Substances

Antibodies, Monoclonal
Antigens, Protozoan