Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines

Laura Gosa; Lisa Ta; David A Nathanson

doi:10.1007/978-1-4939-8935-5_34

Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines

Methods Mol Biol. 2019:1897:425-431. doi: 10.1007/978-1-4939-8935-5_34.

Authors

Laura Gosa^{1

2}, Lisa Ta¹, David A Nathanson^{3

4}

Affiliations

¹ Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
² Ahmanson Translational Imaging Division, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
³ Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA. dnathanson@mednet.ucla.edu.
⁴ Ahmanson Translational Imaging Division, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA. dnathanson@mednet.ucla.edu.

PMID: 30539462
DOI: 10.1007/978-1-4939-8935-5_34

Abstract

Patient tumor tissue processing is an important step in the generation of clinically relevant specimens for in vitro and in vivo studies. Proper disassociation and tissue sample cleanup is a multistep, time-consuming process that ultimately effects the generation of patient derived xenografts and neurosphere cultures. Here we describe a detailed protocol on how to process and disassociate patient glioma tissue and subsequent steps on orthotopic implantation and in vitro generation of neurospheres.

Keywords: Brain tumor digestion; Enzymatic digestion; Neurospheres; Orthotopic xenografts; Patient derived xenografts; Tissue digestion; Tissue disassociation; Tumor processing.

Publication types

Review

MeSH terms

Animals
Biological Specimen Banks / trends*
Cell Line, Tumor*
Humans
Mice
Neoplasms / genetics*
Neoplasms / pathology
Specimen Handling / methods*
Xenograft Model Antitumor Assays

Grants and funding

R01 CA227089/CA/NCI NIH HHS/United States