Evaluation of antioxidant activities of various solvent extract from Sargassum serratifolium and its major antioxidant components

Food Chem. 2019 Apr 25:278:178-184. doi: 10.1016/j.foodchem.2018.11.058. Epub 2018 Nov 10.

Abstract

Sargassum serratifolium has been known to contain a high level of meroterpenoids as antioxidant components. We investigated antioxidant activities and active components in various solvent extracts from S. serratifolium. Ethyl acetate, ethanol, and methanol extracts showed relatively strong DPPH, ABTs, and superoxide radical scavenging activities. Hexane and ethyl acetate extract showed the strongest hydroxyl radical and reactive oxygen species (ROS), respectively, scavenging activities. Sargahydroquinoic acid (SHQA), sargachromanol (SCM) and sargaquinoic acid (SQA) were main antioxidant components in S. serratifolium. Ethanol extract showed the highest levels of SHQA, SCM, and SQA which comprised to be 227 ± 6.31 mg/g. SHQA and SCM exhibited stronger antioxidant capacities than SQA based on lower IC50 values in ROS, DPPH, ABTs, and superoxide radical scavenging assays. The result showed that ethanol is the most efficient extracting solvent for the active components from S. serratifolium and the plant has the potential as a natural antioxidant.

Keywords: Antioxidant activity; Sargassum serratifolium; Solvent extraction. meroterpenoids.

MeSH terms

  • Acetates / chemistry
  • Alkenes / analysis
  • Alkenes / metabolism
  • Animals
  • Antioxidants / analysis*
  • Antioxidants / metabolism*
  • Antioxidants / pharmacology
  • Benzoquinones / analysis
  • Benzoquinones / metabolism
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology
  • Hexanes / chemistry
  • Methanol / chemistry
  • Mice
  • RAW 264.7 Cells
  • Reactive Oxygen Species / metabolism
  • Sargassum / chemistry*
  • Seaweed / chemistry
  • Solvents / chemistry

Substances

  • Acetates
  • Alkenes
  • Antioxidants
  • Benzoquinones
  • Free Radical Scavengers
  • Hexanes
  • Reactive Oxygen Species
  • Solvents
  • sargahydroquinoic acid
  • sargaquinoic acid
  • n-hexane
  • ethyl acetate
  • Methanol