CTHRC1 overexpression promotes cervical carcinoma progression by activating the Wnt/PCP signaling pathway

Oncol Rep. 2019 Mar;41(3):1531-1538. doi: 10.3892/or.2019.6963. Epub 2019 Jan 10.

Abstract

The tumorigenesis and metastasis of tumors are associated with human collagen triple helix repeats containing 1 (CTHRC1). To study the effects and possible impacting mechanisms of CTHRC1 on human cervical carcinoma development, samples of paraffin‑embedded cervical carcinoma and HeLa cells were examined. Immunofluorescence, cell wound scratch assay, western blot analysis and Transwell invasion assay were used to evaluate HeLa cells in response to silencing of the CTHRC1 gene in cervical carcinoma. The expression levels of gap‑associated proteins of the Wnt/PCP pathway in paraffin‑embedded cervical carcinoma samples were also evaluated by immunohistochemical staining. CTHRC1 promoted the migration and invasion of HeLa cells in vitro, downregulated Ror2 and p‑c‑Jun and activated the Wnt/PCP pathway. Furthermore, the expression of p‑c‑Jun, Ror2 and Wnt5a was increased after overexpression of CTHRC1 as revealed in HeLa cells compared to control group. The present experiments revealed that CTHRC1 promoted HeLa cell progression by activating the Wnt/PCP signaling pathway and may play a key role in the invasion and metastasis of cervical carcinoma.

MeSH terms

  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism*
  • Cell Movement
  • Cell Proliferation
  • Cervix Uteri / pathology
  • Disease Progression
  • Down-Regulation
  • Extracellular Matrix Proteins / genetics
  • Extracellular Matrix Proteins / metabolism*
  • Female
  • Gene Expression Regulation, Neoplastic
  • HeLa Cells
  • Humans
  • Middle Aged
  • Prognosis
  • Proto-Oncogene Proteins c-jun / metabolism
  • RNA, Small Interfering
  • Receptor Tyrosine Kinase-like Orphan Receptors / metabolism
  • Survival Analysis
  • Up-Regulation
  • Uterine Cervical Neoplasms / genetics
  • Uterine Cervical Neoplasms / mortality
  • Uterine Cervical Neoplasms / pathology*
  • Wnt Signaling Pathway*
  • Wnt-5a Protein / metabolism

Substances

  • Biomarkers, Tumor
  • CTHRC1 protein, human
  • Extracellular Matrix Proteins
  • Proto-Oncogene Proteins c-jun
  • RNA, Small Interfering
  • WNT5A protein, human
  • Wnt-5a Protein
  • ROR2 protein, human
  • Receptor Tyrosine Kinase-like Orphan Receptors