Effects of exposure to triphenyltin (TPT) contaminant on sperm activity in adulthood of Calomys laucha exposed through breastfeeding

Environ Sci Pollut Res Int. 2019 Mar;26(8):8280-8288. doi: 10.1007/s11356-019-04365-1. Epub 2019 Jan 31.

Abstract

Triphenyltin (TPT) is an organotin compound (OT), primarily used in agriculture and in the composition of antifouling paints for ships worldwide. Studies have showed its effects as an endocrine disrupter in several organisms by preventing enzymatic expression and causing reproductive toxicity. This study aimed to evaluate the effects of exposure to TPT, via breastfeeding, on reproductive physiology in the Calomys laucha species. The experimental design was compound of five groups, two controls and three with different doses of TPT. Moreover, females were exposed by gavage to the TPT for 20 days, from the 1st day postpartum to the 21st postnatal day (PND). Then, the pups were euthanized and the kinetics, organelles, and biochemistry of the sperm were evaluated. The results presented a reduction in total motility in the groups exposed to TPT. Regarding cellular organelles analysis, a loss in membrane integrity was evidenced; the functionality of mitochondria showed diminution followed by increased acrosome reaction. In conclusion, the TPT causes alteration of the reproductive parameters, decreasing the activity and sperm quality in individuals exposed in the breastfeeding phase.

Keywords: Breastfeeding; Sperm activity; Spermatic kinetics; TPT; Triphenyltin.

MeSH terms

  • Animals
  • Arvicolinae / physiology*
  • Ecotoxicology / methods
  • Endocrine Disruptors / toxicity
  • Female
  • Lactation
  • Male
  • Organotin Compounds / toxicity*
  • Reproduction / drug effects
  • Spermatozoa / drug effects*
  • Spermatozoa / pathology

Substances

  • Endocrine Disruptors
  • Organotin Compounds
  • triphenyltin