High miR-3687 Expression Affects Migratory and Invasive Ability of Oesophageal Carcinoma

Anticancer Res. 2019 Feb;39(2):557-565. doi: 10.21873/anticanres.13148.

Abstract

Background/aim: Basaloid squamous cell carcinoma of the oesophagus (BSCCE) is a variant of oesophageal squamous cell carcinoma (ESCC), that has a more aggressive biological behaviour than that of typical ESCC. miR-3687 has been previously identified to be highly expressed in BSCCE. This study aimed to evaluate the prognostic impact of miR-3687 in ESCC and determine the role of miR-3687 in ESCC motility.

Materials and methods: miR-3687 expression in human ESCC cell lines and in primary tumour samples obtained from patients with ESCC who underwent esophagectomy were analyzed via real-time polymerase chain reaction (PCR). Knockdown and over-expression experiments were conducted with miR-3687 siRNA and miRNA mimic, and the effect on cell proliferation, migration, and invasion was analyzed.

Results: A total of 92 samples were analyzed. High miR-3687 expression was correlated with poor prognosis. miR-3687 upregulation promoted cell migration and invasion.

Conclusion: miR-3687 expression negatively impacts the prognosis of patients with ESCC.

Keywords: basaloid squamous cell carcinoma; miR-3687; microRNA; oesophageal cancer.

MeSH terms

  • Aged
  • Carcinoma, Squamous Cell / metabolism*
  • Cell Differentiation
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Esophageal Neoplasms / metabolism*
  • Esophagectomy
  • Female
  • Gene Expression Profiling*
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Male
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Middle Aged
  • Neoplasm Invasiveness
  • Polymerase Chain Reaction
  • Prognosis
  • RNA, Small Interfering / metabolism

Substances

  • MIRN3687 microRNA, human
  • MicroRNAs
  • RNA, Small Interfering