In vitro somatic embryogenesis ofDalbergia sissoo Roxb. -a multipurpose timber-yielding tree

P Das; S Samantaray; G R Rout; A V Roberts

doi:10.1007/BF01142327

In vitro somatic embryogenesis ofDalbergia sissoo Roxb. -a multipurpose timber-yielding tree

Plant Cell Rep. 1997 May;16(8):578-582. doi: 10.1007/BF01142327.

Authors

P Das¹, S Samantaray¹, G R Rout¹, A V Roberts²

Affiliations

¹ Plant Biotechnology Division, Plant Tissue Culture Laboratory, Regional Plant Resource Centre, 751 015, Bhubaneswar, India.
² Plant Biotechnology Research Unit, Department of Life Sciences, University of East London, Ramford Road, E15 4LZ, London, UK.

PMID: 30727582
DOI: 10.1007/BF01142327

Abstract

Somatic embryogenesis was achieved in callus cultures dervied from 40-day-old semimature zygotic embryos ofDalbergia sissoo on semi-solid Murashige and Skoog (MS) salts and vitamins supplemented with 0.46-1.16 μM kinetin, 6.78-9.04 μM 2,4-dichlorophenoxy acetic acid (2,4-D) and 30 g/1 sucrose. Somatic embryos proliferated rapidly by secondary somatic embryogenesis after transfer to half-strength basal MS medium supplemented with 0.46-1.16 μM kinetin and 6.78-9.04 μM 2,4-D with 2% (w/v) sucrose. The light-green somatic embryos germinated on half-strength MS salts and vitamins supplemented with 0.5 mg/1 abscisic acid and 2% (w/v) sucrose. The developmental stages of somatic embryogenesis were studied by light and scanning electron microscopy.

Keywords: Embryogenesis In vitro; Zygotic embryo.