Evaluation of plasma antimicrobial peptide LL-37 and nuclear factor-kappaB levels in stable chronic obstructive pulmonary disease

Int J Chron Obstruct Pulmon Dis. 2019 Jan 25:14:321-330. doi: 10.2147/COPD.S185602. eCollection 2019.

Abstract

Background: Antimicrobial peptides are effectors of host defence against infection and inflammation and can encourage wound repair.

Objectives: The objectives of this study were to investigate the plasma antimicrobial peptide LL-37 and nuclear factor-kappaB (NF-κB) levels in patients with stable COPD compared with a control group and to highlight their importance in immune inflammation.

Methods: One hundred and thirty-eight stable COPD patients and 33 control subjects were enrolled in the study. The COPD patients were classified into four groups based on FEV1 (groups I-IV) and also divided into "low-risk and high-risk" groups (groups A-B [low risk], C-D [high risk]).

Results: Plasma LL-37 levels were significantly lower while plasma NF-κB levels of the COPD patients were significantly higher than those of the control subjects (P<0.001, both). LL-37 levels were significantly lower in group IV than in groups I, II, and III (P<0.01, all). NF-κB levels were significantly higher in groups III and IV than in groups I and II (P<0.05, both). There was a positive correlation between FEV1 and FEV1/FVC in all COPD patients (r=0.742, P<0.001) and in group D (r=0.741, P<0.001). Furthermore, there was an inverse correlation between LL-37 and NF-κB in both the groups C (r=-0.566, P<0.001) and D (r=-0.694, P<0.001) and group C+D combined (r=-0.593, P<0.001). Furthermore, in group C, LL-37 and FEV1 were positively correlated (r=0.633, P<0.001).

Conclusion: Our study indicated that plasma LL-37 and NF-κB may play an important role in chronic immune inflammation. Decreased LL-37 levels may be particularly high risk for patients in stage IV disease. The role of LL-37 as a target for treatment of the immune system and COPD must be widely evaluated.

Keywords: COPD; antimicrobial peptide LL-37; inflammation; nuclear factor kappaB.

Publication types

  • Comparative Study

MeSH terms

  • Aged
  • Aged, 80 and over
  • Antimicrobial Cationic Peptides / blood*
  • Antimicrobial Cationic Peptides / immunology
  • Biomarkers / blood
  • Case-Control Studies
  • Cathelicidins
  • Female
  • Forced Expiratory Volume
  • Humans
  • Inflammation Mediators / blood*
  • Inflammation Mediators / immunology
  • Lung / physiopathology
  • Male
  • Middle Aged
  • NF-kappa B / blood*
  • NF-kappa B / immunology
  • Prognosis
  • Prospective Studies
  • Pulmonary Disease, Chronic Obstructive / blood*
  • Pulmonary Disease, Chronic Obstructive / diagnosis
  • Pulmonary Disease, Chronic Obstructive / immunology
  • Pulmonary Disease, Chronic Obstructive / physiopathology
  • Risk Factors
  • Severity of Illness Index
  • Vital Capacity

Substances

  • Antimicrobial Cationic Peptides
  • Biomarkers
  • Inflammation Mediators
  • NF-kappa B
  • Cathelicidins