Objective: To analyze the relationship between the level of microRNA-29b in circulation and left ventricular hypertrophy in hypertensive patients. Methods: A total of 240 subjects from Henan Province People's Hospital from June 2015 to June 2018 were included in the present study. Among them, 160 were hospitalized patients, and were divided into two groups. Patients with simple hypertension and had no left ventricular hypertrophy (80 cases) were in the simple hypertension group (HBP-NLVH), and patients with hypertension combined with left ventricular hypertrophy (80 cases) were in the high blood pressure with left ventricular hypertrophy group (HBP-LVH). Normal control subjects (80 cases) were those with no hypertension and randomly selected from the medical center of Henan Province People's Hospital. Serum microRNA-29b expressions were detected by real time fluorescence quantitative PCR. The thickness of interventricular septum (IVSD) and left ventricular posterior wall thickness (LVPWD) were measured by echocardiography. Results: Compared with the normal control group (1.95±0.79), the relative expression of microRNA-29b in the patients both in the HBP-NLVH group (2.67±0.92) and the HBP-LVH group (5.12±1.23) was up-regulated, and the difference between normal control and patients was statistically significant (P<0.05). In patients, the microRNA-29b level in the HBP-LVH group was significantly higher than that in the HBP-NLVH group (P<0.05). The expression level of microRNA-29b was positively correlated with IVSD (r=0.71, P<0.05) and LVPWD (r=0.74, P<0.05), respectively. The sensitivity and specificity of serum microRNA-29b levels in the diagnosis of left ventricular hypertrophy in hypertension patients were 96.8% and 91.3%, respectively. Conclusion: Serum microRNA-29b level is elevated in hypertensive patients with left ventricular hypertrophy, and is positively correlated with left ventricular hypertrophy. The circulation microRNA-29b might be a useful biomarker with prognostic value in left ventricular hypertrophy in hypertension patients.
目的： 分析并研究循环中microRNA-29b表达水平变化与高血压患者左心室肥厚之间的关系。 方法： 选取河南省人民医院2015年6月—2018年6月心血管内科住院患者共160例，其中单纯性高血压患者（无左心室肥厚）80例，即单纯高血压组（HBP-NLVH组）；以及高血压合并左心室肥厚患者80例，即高血压伴左心室肥厚组（HBP-LVH组）。随机选取此段时间来河南省人民医院体检中心进行体检无高血压患者80例，即正常对照组，对此240例患者进行研究。运用实时荧光定量PCR来检测此240例患者血清的微小RNA-29b水平；同时心脏超声测定此240例患者的室间隔厚度（IVSD）和左心室后壁厚度（LVPWD）。 结果： 循环中微小RNA-29b的相对表达量在HBP-NLVH组（2.67±0.92）和HBP-LVH组（5.12±1.23）明显高于正常对照组（1.95±0.79），差异均具有显著统计学意义（P值均<0.05）；HBP-NLVH组明显低于HBP-LVH组，差异具有统计学意义（P<0.05）。微小RNA-29b的表达水平与IVSD和LVPWD呈正相关，相关系数r值分别为0.71和0.74，且差异均有统计学意义（P<0.05）。循环微小RNA-29b在高血压伴左心室肥厚诊断中的敏感度和特异度分别为96.8%和91.3%。 结论： 微小RNA-29b在高血压伴左心室肥厚患者循环中特异性的高表达，与高血压左心室肥厚呈正相关，且在诊断高血压左心室肥厚有较好的敏感性和特异性，为筛查左心室肥厚提供了新的切入点。.
Keywords: Hypertension; Left ventricular hypertrophy; MicroRNA-29b.