Twenty-nine species of fungi were isolated from normal and discolored seeds of barley. More species were isolated from discolored seeds and percentage of occurrence of individual fungi was higher in nondisinfected seeds as compared to disinfected seeds. The most common genera found were Ulocladium (four species); Alternaria , Aspergillus , and Drechslera (three species); Curvularia , Fusarium , Mucor , Penicillium , and Syncephalastrum (two species). Diathane was found to be most effective among fungicides tested.