Draft Genome Sequence of a bla NDM-1- and bla PME-1-Harboring Pseudomonas aeruginosa Clinical Isolate from Pakistan

Sidra Irum; Robert F Potter; Rubina Kamran; Zeeshan Mustafa; Meghan A Wallace; C A Burnham; Gautam Dantas; Saadia Andleeb

doi:10.1128/MRA.00107-19

Draft Genome Sequence of a bla _NDM-1- and bla _PME-1-Harboring Pseudomonas aeruginosa Clinical Isolate from Pakistan

Microbiol Resour Announc. 2019 Apr 25;8(17):e00107-19. doi: 10.1128/MRA.00107-19.

Authors

Sidra Irum¹, Robert F Potter², Rubina Kamran³, Zeeshan Mustafa¹, Meghan A Wallace⁴, C A Burnham^{4

5

6}, Gautam Dantas^{2

4

6

7}, Saadia Andleeb⁸

Affiliations

¹ Atta-Ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad, Pakistan.
² The Edison Family Center for Genome Sciences and Systems Biology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
³ Pakistan Institute of Medical Sciences (PIMS), Islamabad, Pakistan.
⁴ Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
⁵ Department of Pediatrics, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
⁶ Department of Molecular Microbiology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
⁷ Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri, USA.
⁸ Atta-Ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad, Pakistan saadia.andleeb@asab.nust.edu.pk.

Abstract

We performed Illumina whole-genome sequencing on a carbapenem-resistant Pseudomonas aeruginosa strain isolated from a cystic fibrosis patient with chronic airway colonization. The draft genome comprises 6,770,411 bp, including the carbapenemase bla _NDM-1 and the extended-spectrum beta-lactamase bla _PME-1 This isolate harbors 3 prophages, 14 antibiotic resistance genes, and 257 virulence genes.

Abstract

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