Mesenchymal stem cells-curcumin loaded chitosan nanoparticles hybrid vectors for tumor-tropic therapy

Int J Biol Macromol. 2019 Aug 1:134:1002-1012. doi: 10.1016/j.ijbiomac.2019.04.201. Epub 2019 May 4.

Abstract

The combination of controlled release technology and targeted drug delivery has become a promising strategy for cancer therapy. In this study, cell-nanoparticle hybrid vector was constructed using mesenchymal stem cells as the targeting cellular carrier and biotinylated chitosan polymer nanoparticles as the drug depot. Drug-loaded nanoparticles (hydrodynamic size =377.0 ± 14.6 nm and zeta potential = 9.6 ± 1.9 mV) were prepared by encapsulating hydrophobic model drug curcumin into biotinylated chitosan polymer. The biotin-modified nanoparticles were anchored on biotinylated mesenchymal stem cells surface by biotin-avidin binding, achieving an upload of 54.73 ± 3.95 pg/cell. The anchorage of nanoparticles on mesenchymal stem cells had no effect on their viability and homing property. Biotin-avidin binding lasted over 48 h, which could be sufficient for cell-directed tumor-tropic delivery. The in vitro and in vivo anti-tumor results advocate that cell-nanoparticle hybrid vector could prove beneficial in pulmonary melanoma metastasis therapy.

Keywords: Biotinylated chitosan nanoparticles; Hybrid vector; Mesenchymal stem cells.

MeSH terms

  • Animals
  • Biotin
  • Cell Movement / drug effects
  • Cell Survival / drug effects
  • Chitosan* / chemistry
  • Curcumin / administration & dosage*
  • Curcumin / chemistry
  • Disease Models, Animal
  • Drug Carriers* / chemistry
  • Drug Delivery Systems
  • Drug Liberation
  • Fluorescent Antibody Technique
  • Male
  • Mesenchymal Stem Cell Transplantation* / methods
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / metabolism*
  • Mice
  • Nanoparticles* / chemistry
  • Nanoparticles* / ultrastructure
  • Neoplasms / therapy
  • Polymers
  • Tumor Burden / drug effects
  • Xenograft Model Antitumor Assays

Substances

  • Drug Carriers
  • Polymers
  • Biotin
  • Chitosan
  • Curcumin