HLA-G expression during hookworm infection in pregnant women

Acta Trop. 2019 Aug:196:52-59. doi: 10.1016/j.actatropica.2019.04.026. Epub 2019 May 9.

Abstract

Introduction: HLA-G plays a key role on immune tolerance. Pathogens can induce soluble HLA-G (sHLA-G) production to down-regulate the host immune response, creating a tolerogenic environment favorable for their dissemination. To our knowledge, no study has yet been conducted to assess the relationship between sHLA-G and geohelminth infections.

Methods: The study was conducted in Allada, Southeastern Benin, from 2011-2014. The study population encompassed 400 pregnant women, included before the end of the 28th week of gestation and followed-up until delivery. At two antenatal care visits and at delivery, stool and blood samples were collected. Helminths were diagnosed by means of the Kato-Katz concentration technique. We used quantile regression to analyze the association between helminth infections and sHLA-G levels during pregnancy.

Results: sHLA-G levels gradually increased during pregnancy and reached maximal levels at delivery. Prevalence of helminth infections was low, with a majority of hookworm infections. We found significantly more hookworm-infected women above the 80th quantile (Q80) of the distribution of the mean sHLA-G level (p < 0.03, multivariate quantile regression). Considering only women above the Q80 percentile, the mean sHLA-G level was significantly higher in hookworm-infected compared to uninfected women (p = 0.04).

Conclusion: High levels of sHLA-G were associated with hookworm infection in pregnant women. This result is consistent with the potential involvement of sHLA-G in immune tolerance induced by helminths during pregnancy.

Keywords: HLA-G; Helminth; Hookworm; Immune tolerance; Neglected tropical diseases; Pregnant women.

MeSH terms

  • Adult
  • Benin / epidemiology
  • Female
  • HLA-G Antigens / genetics
  • HLA-G Antigens / metabolism*
  • Hookworm Infections / epidemiology
  • Hookworm Infections / immunology
  • Hookworm Infections / metabolism*
  • Humans
  • Pregnancy
  • Pregnancy Complications, Parasitic / epidemiology
  • Pregnancy Complications, Parasitic / metabolism*
  • Prevalence
  • Young Adult

Substances

  • HLA-G Antigens