Abstract
PEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased after PEAR1 overexpression and decreased upon its suppression. Meanwhile, we found that, upon PEAR1 induction, both the expression of the focal adhesion-associated and MyoG, and the myotube fusion rate increased. However, when PEAR1 was suppressed, opposite results were obtained. Immunoprecipitation revealed an association between PEAR1 and ITGB1. Notably, inhibition of FAK and ITGB1 repressed cell differentiation. In conclusion, our study indicated that PEAR1 is involved in the regulation of bovine MDSC migration and differentiation.
Keywords:
PEAR1; differentiation; focal adhesion kinase; integrin beta-1; migration.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cattle
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Cell Differentiation*
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Cell Movement*
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Cells, Cultured
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Focal Adhesion Kinase 1 / genetics
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Focal Adhesion Kinase 1 / metabolism*
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Focal Adhesions
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Integrin beta1 / genetics
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Integrin beta1 / metabolism*
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Myoblasts / cytology
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Myoblasts / physiology*
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Phosphorylation
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Platelet Aggregation
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism*
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Satellite Cells, Skeletal Muscle / cytology
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Satellite Cells, Skeletal Muscle / physiology*
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Signal Transduction
Substances
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Integrin beta1
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Receptors, Cell Surface
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Focal Adhesion Kinase 1
Grants and funding
This work was supported by the breeding program for high-quality new varieties of genetically modified bovine from the National Natural Science Fund Youth Science Fund Project[grant number [31801150] and Heilongjiang Natural Science Foundation[C2018031];the breeding program for high-quality new varieties of genetically modified bovine from the National Natural Science Fund Youth Science Fund Project [31801150].