Molecular cloning of Bacillus subtilis genes involved in DNA metabolism

M Perego; E Ferrari; M T Bassi; A Galizzi; P Mazza

doi:10.1007/BF00329829

Molecular cloning of Bacillus subtilis genes involved in DNA metabolism

Mol Gen Genet. 1987 Aug;209(1):8-14. doi: 10.1007/BF00329829.

Authors

M Perego¹, E Ferrari, M T Bassi, A Galizzi, P Mazza

Affiliation

¹ Dipartimento di Genetica e Microbiologia, A. Buzzati-Traverso, Università di Pavia, Italy.

PMID: 3118144
DOI: 10.1007/BF00329829

Abstract

Different clones carrying a chromosomal DNA fragment able to transform Bacillus subtilis mutants dnaA13, dnaB19, dnaG5, recG40 and polA42 to a wild-type phenotype were isolated from a library constructed in plasmid pJH101. A lambda recombinant clone carrying a chromosomal fragment able to transform dnaC mutants was obtained from a lambda Charon 4A library. A restriction map of the cloned DNA fragments was constructed. The 11.3 kb cloned DNA fragment of plasmid pMP60-13 containing the wild-type sequence of dnaG5 was shown to transform a recF33 mutant as well.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / genetics*
Bacillus subtilis / metabolism
Cloning, Molecular*
DNA, Bacterial / metabolism*
Genes*
Genes, Bacterial*
Genotype
Mutation
Plasmids

Substances

DNA, Bacterial